Open AccessVisualizing human photoreceptor and retinal pigment epithelium cell mosaics in a single volume scan over an extended field of view with adaptive optics optical coherence tomography
Author(s) -
Muhammad Faizan Shirazi,
Elisabeth Brunner,
Marie Laslandes,
Andreas Pollreisz,
Christoph K. Hitzenberger,
Michael Pircher
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.393906
Subject(s) - retinal pigment epithelium , optical coherence tomography , optics , adaptive optics , wavefront , human eye , retinal , preclinical imaging , retina , field of view , lens (geology) , coherence (philosophical gambling strategy) , physics , computer science , biology , ophthalmology , medicine , in vivo , microbiology and biotechnology , quantum mechanics
Using adaptive optics optical coherence tomography, human photoreceptors and retinal pigment epithelium (RPE) cells are typically visualized on a small field of view of ∼1° to 2°. In addition, volume averaging is required for visualizing the RPE cell mosaic. To increase the imaging area, we introduce a lens based spectral domain AO-OCT system that shows low aberrations within an extended imaging area of 4°×4° while maintaining a high (theoretical) transverse resolution (at >7 mm pupil diameter) in the order of 2 µm. A new concept for wavefront sensing is introduced that uses light mainly originating from the RPE layer and yields images of the RPE cell mosaic in a single volume acquisition. The capability of the instrument for in vivo imaging is demonstrated by visualizing various cell structures within the posterior retinal layers over an extended field of view.