Open AccessHigh-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip
Author(s) -
Federico Sala,
Michele Castriotta,
Petra Paiè,
Andrea Farina,
Sarah D’Annunzio,
Alessio Zippo,
Roberto Osellame,
Francesca Bragheri,
Andrea Bassi
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.393892
Subject(s) - light sheet fluorescence microscopy , microscopy , fluorescence microscope , throughput , microscope , fluorescence lifetime imaging microscopy , microfluidics , materials science , fluorescence , photoactivated localization microscopy , optical microscope , microchannel , optics , biomedical engineering , nanotechnology , super resolution microscopy , computer science , scanning electron microscope , medicine , telecommunications , physics , composite material , wireless
Single-cell analysis techniques are fundamental to study the heterogeneity of cellular populations, which is the basis to understand several biomedical mechanisms. Light-sheet fluorescence microscopy is a powerful technique for obtaining high-resolution imaging of individual cells, but the complexity of the setup and the sample mounting procedures limit its overall throughput. In our work, we present an optofluidic microscope-on-chip with integrated microlenses for light-sheet shaping and with a fluidic microchannel that allows the automatic and continuous delivery of samples of a few tens of microns in size. The device is used to perform dual-color fluorescence analysis and 3D reconstruction of xenograft-derived mouse breast cancer cells.