Open AccessImaging intracellular motion with dynamic micro-optical coherence tomography
Author(s) -
Hui Min Leung,
Michelle L Wang,
Hany Osman,
Elham Abouei,
Calum MacAulay,
Michele Follen,
Joseph A. Gardecki,
Guillermo J. Tearney
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.390782
Subject(s) - optical coherence tomography , intracellular , coherence (philosophical gambling strategy) , optics , pixel , contrast (vision) , tomography , computer science , physics , biology , microbiology and biotechnology , quantum mechanics
This paper describes a new technology that uses 1-µm-resolution optical coherence tomography (µOCT) to obtain cross-sectional images of intracellular dynamics with dramatically enhanced image contrast. This so-called dynamic µOCT (d-µOCT) is accomplished by acquiring a time series of µOCT images and conducting power frequency analysis of the temporal fluctuations that arise from intracellular motion on a pixel-per-pixel basis. Here, we demonstrate d-µOCT imaging of freshly excised human esophageal and cervical biopsy samples. Depth-resolved d-µOCT images of intact tissue show that intracellular dynamics provides a new contrast mechanism for µOCT that highlights subcellular morphology and activity in epithelial surface maturation patterns.