Open AccessEmphysema quantified: mapping regional airway dimensions using 2D phase contrast X-ray imaging
Author(s) -
Marcus J. Kitchen,
Genevieve Buckley,
Lauren Kerr,
Katie Lee,
Kentaro Uesugi,
Naoto Yagi,
Stuart B. Hooper
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.390587
Subject(s) - phase contrast imaging , receiver operating characteristic , contrast (vision) , airway , nuclear medicine , analyser , sensitivity (control systems) , materials science , attenuation , phase contrast microscopy , optics , x ray , scattering , biomedical engineering , medicine , physics , surgery , electronic engineering , engineering
We have developed an analyser-based phase contrast X-ray imaging technique to measure the mean length scale of pores or particles that cannot be resolved directly by the system. By combining attenuation, phase and ultra-small angle X-ray scattering information, the technique was capable of measuring differences in airway dimension between lungs of healthy mice and those with mild and severe emphysema. Our measurements of airway dimensions from 2D images showed a 1:1 relationship to the actual airway dimensions measured using micro-CT. Using 80 images, the sensitivity and specificity were measured to be 0.80 and 0.89, respectively, with the area under the ROC curve close to ideal at 0.96. Reducing the number of images to 11 slightly decreased the sensitivity to 0.75 and the ROC curve area to 0.90, whilst the specificity remained high at 0.89.