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Label-free redox imaging of patient-derived organoids using selective plane illumination microscopy
Author(s) -
Peter F. Favreau,
Jiaye He,
Daniel A. Gil,
Dustin A. Deming,
Jan Huisken,
Melissa C. Skala
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.389164
Subject(s) - organoid , autofluorescence , microscopy , biological imaging , fluorescence microscope , fluorescence lifetime imaging microscopy , endogeny , fluorescence , chemistry , nanotechnology , materials science , medicine , pathology , biology , microbiology and biotechnology , optics , biochemistry , physics
High-throughput drug screening of patient-derived organoids offers an attractive platform to determine cancer treatment efficacy. Here, selective plane illumination microscopy (SPIM) was used to determine treatment response in organoids with endogenous fluorescence from the metabolic coenzymes NAD(P)H and FAD. Rapid 3-D autofluorescence imaging of colorectal cancer organoids was achieved. A quantitative image analysis approach was developed to segment each organoid and quantify changes in endogenous fluorescence caused by treatment. Quantitative analysis of SPIM volumes confirmed the sensitivity of patient-derived organoids to standard therapies. This proof-of-principle study demonstrates that SPIM is a powerful tool for high-throughput screening of organoid treatment response.

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