Open AccessRealization of a time-correlated photon counting technique for fluorescence analysis
Author(s) -
Lei Dai,
Jian Liu,
Kun Liang,
Ronghua Yang,
Dan Han,
Bo Lu
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.385870
Subject(s) - photon counting , photomultiplier , photon , optics , physics , silicon photomultiplier , fluorescence , photon antibunching , dynamic range , detection limit , fluorescence correlation spectroscopy , mathematics , statistics , detector , scintillator
An ultralow level light detection module, the time-correlated photon counter, is proposed and evaluated for fluorescence analysis. The time-correlated photon counter employs a silicon photomultiplier as a photon counting sensor in conjunction with a Poisson statistics algorithm and a double time windows technique, and therefore it can accurately count the photon number. The time-correlated photon counter is compatible with the time-correlated single photon counting technique and can record the arrival time of very faint light signals. This low-cost and compact instrument was used to analyze the intensity and lifetime of fluorescein isothiocyanate; a limit of detection of 16 pg/ml with a large linear dynamic range from 2.86 pg/ml to 0.5 µg/ml was obtained, and the lifetime of fluorescein isothiocyanate was measured to be 3.758 ns, which agrees well with the results of a sophisticated commercial fluorescence analysis instrument. The time-correlated photon counter may be useful in applications such as point-of-care testing.