Open AccessDesign of a high-resolution light field miniscope for volumetric imaging in scattering tissue
Author(s) -
Yanqin Chen,
Bo Xiong,
Yujia Xue,
Xin Jin,
Joseph Greene,
Lei Tian
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.384673
Subject(s) - point spread function , monte carlo method , optics , image resolution , robustness (evolution) , scattering , light field , computer science , field of view , light scattering , medical imaging , biophotonics , computer vision , artificial intelligence , physics , photonics , mathematics , biochemistry , statistics , chemistry , gene
Integrating light field microscopy techniques with existing miniscope architectures has allowed for volumetric imaging of targeted brain regions in freely moving animals. However, the current design of light field miniscopes is limited by non-uniform resolution and long imaging path length. In an effort to overcome these limitations, this paper proposes an optimized Galilean-mode light field miniscope (Gali-MiniLFM), which achieves a more consistent resolution and a significantly shorter imaging path than its conventional counterparts. In addition, this paper provides a novel framework that incorporates the anticipated aberrations of the proposed Gali-MiniLFM into the point spread function (PSF) modeling. This more accurate PSF model can then be used in 3D reconstruction algorithms to further improve the resolution of the platform. Volumetric imaging in the brain necessitates the consideration of the effects of scattering. We conduct Monte Carlo simulations to demonstrate the robustness of the proposed Gali-MiniLFM for volumetric imaging in scattering tissue.