Open AccessOsteoblast differentiation of bone marrow stromal cells by femtosecond laser bone ablation
Author(s) -
Jukuan Zheng,
Xinyue Zhang,
Yaopeng Zhang,
Fusong Yuan
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.383721
Subject(s) - alkaline phosphatase , osteocalcin , stromal cell , runx2 , ablation , osteoblast , laser ablation , mesenchymal stem cell , femtosecond , chemistry , pathology , microbiology and biotechnology , laser , materials science , biomedical engineering , biology , medicine , optics , biochemistry , physics , in vitro , enzyme
This study examines the osteogenic effect of femtosecond laser bone ablation on bone mesenchymal stromal cells (BMSCs). Three-week old Sprague-Dawley (SD) rats were selected for experiments. Right tibias were ablated by a 10-W femtosecond laser (treated group), whereas left tibias were not subjected to laser ablation (control group). After ablation, BMSCs of both tibias were cultured and purified separately. Cell proliferation was then analyzed, as well as the expressions of RNA and several proteins (alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and osteocalcin (OCN)). The results suggest that femtosecond laser ablation promotes the differentiation of BMSCs and up-regulates the expression of ALP, RUNX2, and OCN, without affecting BMSC proliferation.