Open AccessVascular bifurcation mapping with photoacoustic microscopy
Author(s) -
Boudewijn van der Sanden,
Olivier Hugon,
Mehdi Inglebert,
Olivier Jacquin,
Éric Lacot
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.383583
Subject(s) - bifurcation , photoacoustic imaging in biomedicine , biomarker , biomedical engineering , radiology , optics , medicine , biology , physics , nonlinear system , biochemistry , quantum mechanics
The early detection of microvascular changes in cancer diagnosis is needed in the clinic. A change in the vascular bifurcation density is a biomarker for the sprouting activity. Here, O ptical- R esolution P hoto A coustic M icroscopy is used for quantitative vascular bifurcation mapping in 2D after the creation of Vi rtual Tu bes out of Bi furcations. In stacks of OR-PAM images of the hemoglobin distribution, bifurcations become tubes and are selected by the 3D tubeness filter. These fast analyses will be compared to a classical approach and are easier to implement for functional analysis of the vascular bifurcation density in healthy and diseased tissues.