Open AccessQuantitative phase imaging of stromal prognostic markers in pancreatic ductal adenocarcinoma
Author(s) -
Michael J. Fanous,
Adib Keikhosravi,
Andre KajdacsyBalla,
Kevin W. Eliceiri,
Gabriel Popescu
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.383242
Subject(s) - pancreatic ductal adenocarcinoma , pathology , stromal cell , microscopy , biopsy , medicine , pancreas , adenocarcinoma , pancreatic cancer , cancer
New quantitative prognostic markers are needed for improved pancreatic ductal adenocarcinoma (PDAC) prognosis. Second harmonic generation microscopy has been used to show that collagen fiber alignment in PDAC is a negative prognostic factor. In this work, a series of PDAC and normal adjacent tissue (NAT) biopsies were imaged with spatial light interference microscopy (SLIM). Quantitative analysis performed on the biopsy SLIM images show that PDAC fiber structures have lower alignment per unit length, narrower width, and are longer than NAT controls. Importantly, fibrillar collagen in PDAC shows an inverse relationship between survival data and fiber width and length (p < 0.05).