Open AccessTowards real-time wide-field fluorescence lifetime imaging of 5-ALA labeled brain tumors with multi-tap CMOS cameras
Author(s) -
David P. Reichert,
Mikael T. Erkkilä,
Gerhard A. Holst,
Nancy HeckerDenschlag,
Marco Wilzbach,
Christoph Hauger,
Wolfgang Drexler,
Johanna Gesperger,
Barbara Kiesel,
Thomas Roetzer,
Angelika Unterhuber,
Georg Widhalm,
Rainer A. Leitgeb,
Marco Andreana
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.382817
Subject(s) - fluorescence lifetime imaging microscopy , fluorescence , neuroimaging , cmos sensor , sensitivity (control systems) , materials science , biomedical engineering , cmos , computer science , optics , physics , medicine , optoelectronics , psychiatry , engineering , electronic engineering
Fluorescence guided neurosurgery based on 5-aminolevulinic acid (5-ALA) has significantly increased maximal safe resections. Fluorescence lifetime imaging (FLIM) of 5-ALA could further boost this development by its increased sensitivity. However, neurosurgeons require real-time visual feedback which was so far limited in dual-tap CMOS camera based FLIM. By optimizing the number of phase frames required for reconstruction, we here demonstrate real-time 5-ALA FLIM of human high- and low-grade glioma with up to 12 Hz imaging rate over a wide field of view (11.0 x 11.0 mm). Compared to conventional fluorescence imaging, real-time FLIM offers enhanced contrast of weakly fluorescent tissue.