Open AccessTowards real-time wide-field fluorescence lifetime imaging of 5-ALA labeled brain tumors with multi-tap CMOS cameras
Author(s) -
David Reichert,
Mikael T. Erkkilä,
Gerhard Holst,
Nancy HeckerDenschlag,
Marco Wilzbach,
Christoph Hauger,
Wolfgang Drexler,
Johanna Gesperger,
Barbara Kiesel,
Thomas Roetzer-Pejrimovsky,
Angelika Unterhuber,
Georg Widhalm,
Rainer A. Leitgeb,
Marco Andreana
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.382817
Subject(s) - fluorescence lifetime imaging microscopy , fluorescence , neuroimaging , cmos sensor , sensitivity (control systems) , glioma , materials science , biomedical engineering , computer science , cmos , optics , physics , medicine , optoelectronics , electronic engineering , psychiatry , engineering , cancer research
Fluorescence guided neurosurgery based on 5-aminolevulinic acid (5-ALA) has significantly increased maximal safe resections. Fluorescence lifetime imaging (FLIM) of 5-ALA could further boost this development by its increased sensitivity. However, neurosurgeons require real-time visual feedback which was so far limited in dual-tap CMOS camera based FLIM. By optimizing the number of phase frames required for reconstruction, we here demonstrate real-time 5-ALA FLIM of human high- and low-grade glioma with up to 12 Hz imaging rate over a wide field of view (11.0 x 11.0 mm). Compared to conventional fluorescence imaging, real-time FLIM offers enhanced contrast of weakly fluorescent tissue.