MusiJ: an ImageJ plugin for video nanoscopy | Zendy

Sebastián Acuña | Zendy; Florian Ströhl | Zendy; Ida S. Opstad | Zendy; Balpreet Singh Ahluwalia | Zendy; Krishna Agarwal | Zendy

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MusiJ: an ImageJ plugin for video nanoscopy

Author(s) -

Sebastián Acuña,

Florian Ströhl,

Ida S. Opstad,

Balpreet Singh Ahluwalia,

Krishna Agarwal

Publication year - 2020

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.382735

Subject(s) - plug in , computer science , computer graphics (images) , programming language

We present an open-source implementation of the fluctuation-based nanoscopy method MUSICAL for ImageJ. This implementation improves the algorithm's computational efficiency and takes advantage of multi-threading to provide orders of magnitude faster reconstructions than the original MATLAB implementation. In addition, the plugin is capable of generating super-resolution videos from large stacks of time-lapse images via an interleaved reconstruction, thus enabling easy-to-use multi-color super-resolution imaging of dynamic systems.

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