Open AccessVisualization of blood cell contrast in nailfold capillaries with high-speed reverse lens mobile phone microscopy
Author(s) -
Gregory N. McKay,
Nela Mohan,
I. Butterworth,
Aurélien Bourquard,
Álvaro SánchezFerro,
Carlos Castro-González,
Nicholas J. Durr
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.382376
Subject(s) - contrast (vision) , optics , materials science , biomedical engineering , computer science , microscopy , high contrast , absorption (acoustics) , medicine , artificial intelligence , physics
Quantification of optical absorption gaps in nailfold capillaries has recently shown promise as a non-invasive technique for neutropenia screening. Here we demonstrate a low-cost, portable attachment to a mobile phone that can resolve optical absorption gaps in nailfold capillaries using a reverse lens technique and oblique 520nm illumination. Resolution <4 μ m within a 1mm 2 on-axis region is demonstrated, and wide field of view (3.5mm × 4.8mm) imaging is achieved with resolution <6 μ m in the periphery. Optical absorption gaps (OAGs) are visible in superficial capillary loops of a healthy human participant by an ∼8-fold difference in contrast-to-noise ratio with respect to red blood cell absorption contrast. High speed video capillaroscopy up to 240 frames per second (fps) is possible, though 60fps is sufficient to resolve an average frequency of 37 OAGs/minute passing through nailfold capillaries. The simplicity and portability of this technique may enable the development of an effective non-invasive tool for white blood cell screening in point-of-care and global health settings.