Open AccessThree-dimensional label-free observation of individual bacteria upon antibiotic treatment using optical diffraction tomography
Author(s) -
Jeong-hun Oh,
Jea Sung Ryu,
Moosung Lee,
JinWoo Jung,
Sang Youn Han,
Hyun Jung Chung,
Yong Keun Park
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.377740
Subject(s) - antibiotics , bacteria , ampicillin , microbiology and biotechnology , bacillus subtilis , tomography , escherichia coli , optics , biology , physics , biochemistry , genetics , gene
Measuring alterations in bacteria upon antibiotic application is important for basic studies in microbiology, drug discovery, clinical diagnosis, and disease treatment. However, imaging and 3D time-lapse response analysis of individual bacteria upon antibiotic application remain largely unexplored mainly due to limitations in imaging techniques. Here, we present a method to systematically investigate the alterations in individual bacteria in 3D and quantitatively analyze the effects of antibiotics. Using optical diffraction tomography, in-situ responses of Escherichia coli and Bacillus subtilis to various concentrations of ampicillin were investigated in a label-free and quantitative manner. The presented method reconstructs the dynamic changes in the 3D refractive-index distributions of living bacteria in response to antibiotics at sub-micrometer spatial resolution.