Open AccessNoninvasive diagnosis of mucopolysaccharidosis via depth-resolved optical spectroscopy of the outer ear
Author(s) -
Richa Mittal,
Philip H. Schwartz,
David J. Brick,
Chad A. Lieber
Publication year - 2011
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.2.002741
Subject(s) - raman spectroscopy , mucopolysaccharidosis , mucopolysaccharidosis i , pathology , glycosaminoglycan , ex vivo , linear discriminant analysis , nuclear magnetic resonance , medicine , chemistry , biomedical engineering , in vivo , optics , biology , anatomy , computer science , disease , artificial intelligence , enzyme replacement therapy , physics , microbiology and biotechnology
Current diagnostics for lysosomal storage disorders such as mucopolysaccharidosis (MPS) rely on evaluation of ex vivo bodily fluids, which has several shortcomings. In this study, we evaluated whether Raman spectroscopy could noninvasively diagnose MPS in a murine model. Via confocal sampling of the murine outer ear, Raman spectra were obtained at multiple depths. Partial least-squares discriminant analysis of the processed Raman spectra showed a 93% sensitivity and 91% specificity for disease. The discriminant algorithm relied on several Raman bands related to glycosaminoglycans (GAGs) that typically accumulate in MPS. These findings indicate the possibility for a new, noninvasive diagnostic tool for MPS.