Open AccessDual-mode emission and transmission microscopy for virtual histochemistry using hematoxylin- and eosin-stained tissue sections
Author(s) -
Farzad Fereidouni,
Austin Todd,
Yuheng Li,
Chih Ching Chang,
Keith Luong,
Avi Rosenberg,
Yong Jae Lee,
James W. Chan,
Alexander D. Borowsky,
Karen Matsukuma,
Kuang-Yu Jen,
Richard M. Levenson
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.006516
Subject(s) - trichrome , masson's trichrome stain , h&e stain , microscopy , pathology , trichrome stain , stain , staining , bright field microscopy , eosin , biomedical engineering , computer science , medicine , immunohistochemistry
In the clinical practice of pathology, trichrome stains are commonly used to highlight collagen and to help evaluate fibrosis. Such stains do delineate collagen deposits but are not molecularly specific and can suffer from staining inconsistencies. Moreover, performing histochemical stain evaluation requires the preparation of additional sections beyond the original hematoxylin- and eosin-stained slides, as well as additional staining steps, which together add cost, time, and workflow complications. We have developed a new microscopy approach, termed DUET (DUal-mode Emission and Transmission) that can be used to extract signals that would typically require special stains or advanced optical methods. Our preliminary analysis demonstrates the potential of using the resulting signals to generate virtual histochemical images that resemble trichrome-stained slides and can support clinical evaluation. We demonstrate advantages of this approach over images acquired from conventional trichrome-stained slides and compare them with images created using second harmonic generation microscopy.