Open AccessGabor domain optical coherence microscopy combined with laser scanning confocal fluorescence microscopy
Author(s) -
Changsik Yoon,
Yue Qi,
Humberto Mestre,
Cristina Canavesi,
Olivia J. Marola,
Andrea Cogliati,
Maiken Nedergaard,
Richard T. Libby,
Jannick P. Rolland
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.006242
Subject(s) - microscopy , confocal , confocal microscopy , optical coherence tomography , fluorescence lifetime imaging microscopy , fluorescence , light sheet fluorescence microscopy , optics , fluorescence microscope , laser scanning , materials science , two photon excitation microscopy , microscope , laser , physics
We report on the development of fluorescence Gabor domain optical coherence microscopy (Fluo GD-OCM), a combination of GD-OCM with laser scanning confocal fluorescence microscopy (LSCFM) for synchronous micro-structural and fluorescence imaging. The dynamic focusing capability of GD-OCM provided the adaptive illumination environment for both modalities without any mechanical movement. Using Fluo GD-OCM, we imaged ex vivo DsRed-expressing cells in the brain of a transgenic mouse, as well as Cy3-labeled ganglion cells and Cy3-labeled astrocytes from a mouse retina. The self-registration of images taken by the two different imaging modalities showed the potential for a correlative study of subjects and double identification of the target.