Geometrical-optics approach to measure the optical density of bacterial cultures using a LED-based photometer | Zendy

Massimiliano Lucidi | Zendy; M. Ajmone Marsan | Zendy; Francesco Pudda | Zendy; Mattia Pirolo | Zendy; Emanuela Frangipani | Zendy; Paolo Visca | Zendy; Gabriella Cincotti | Zendy

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Geometrical-optics approach to measure the optical density of bacterial cultures using a LED-based photometer

Author(s) -

Massimiliano Lucidi,

M. Ajmone Marsan,

Francesco Pudda,

Mattia Pirolo,

Emanuela Frangipani,

Paolo Visca,

Gabriella Cincotti

Publication year - 2019

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.10.005600

Subject(s) - optics , photometer , measure (data warehouse) , stray light , attenuation , physics , computer science , database

We develop a suitable geometrical-optics approach and demonstrate that it is possible to measure the optical density (OD) of bacterial cultures using a light emitting diode (LED)-based photometer. We measure both attenuation and spot-size variation, and we compensate for diffraction and stray-light impairment related to the incoherent source and large detection area. The approach is validated for different concentrations of two bacterial species, Escherichia coli and Staphylococcus aureus , that present different shapes and clustering organization.

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