Open AccessChromophore selective multi-wavelength photoacoustic remote sensing of unstained human tissues
Author(s) -
Saad Abbasi,
Martin Le,
Bazil Sonier,
Kevan Bell,
Deepak Dinakaran,
Gilbert Bigras,
John R. Mackey,
Parsin Haji Reza
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.005461
Subject(s) - microscope , histology , biomedical engineering , microscopy , photoacoustic imaging in biomedicine , materials science , histopathology , medicine , optics , pathology , physics
Identifying positive surgical margins after resection of cancer often triggers re-excision and adjuvant treatments. Incomplete initial resections result in poorer patient outcomes, psychological and financial stress to the patient and increased healthcare costs. Surgical margins are typically assessed post-operatively using time consuming and expensive slide-based histopathology tissue analysis. Currently, a real-time non-contact virtual histology-like intraoperative margin assessment tool is not available. To address this need, we have developed a non-contact multi-wavelength reflection-mode, photoacoustic remote sensing (PARS) microscope demonstrating chromophore selective contrast in human tissues. We show the capabilities of multi-wavelength PARS microscopy utilizing both 266 nm and 532 nm excitation wavelengths and a 1310 nm detection wavelength. Cell nuclei and hemoglobin were visualized at the cellular scale without the addition of exogenous contrast agents. These works provide a critical step towards a virtual histology tool to provide intraoperative histology-like information in living tissue.