Open AccessTransdermal fluorescence detection of a dual fluorophore system for noninvasive point-of-care gastrointestinal permeability measurement
Author(s) -
Richard B. Dorshow,
James R. Johnson,
Martin P. Debreczeny,
I. Rochelle Riley,
Jeng J. Shieh,
Thomas E. Rogers,
Carla Hall-Moore,
Nurmohammad Shaikh,
L. Colleen Rouggly-Nickless,
Phillip I. Tarr
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.005103
Subject(s) - fluorophore , fluorescence , point of care , medicine , intestinal permeability , biomedical engineering , materials science , pathology , optics , physics
The intestinal mucosal barrier prevents macromolecules and pathogens from entering the circulatory stream. Tight junctions in this barrier are compromised in inflammatory bowel diseases, environmental enteropathy, and enteric dysfunction. Dual sugar absorption tests are a standard method for measuring gastrointestinal integrity, however, these are not clinically amenable. Herein, we report on a dual fluorophore system and fluorescence detection instrumentation for which gastrointestinal permeability is determined in a rat small bowel disease model from the longitudinal measured transdermal fluorescence of each fluorophore. This fluorophore technology enables a specimen-free, noninvasive, point-of-care gastrointestinal permeability measurement which should be translatable to human clinical studies.