Open AccessEnhanced resolution 3D digital cytology and pathology with dual-view inverted selective plane illumination microscopy
Author(s) -
Bihe Hu,
Guang Li,
J. Quincy Brown
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.003833
Subject(s) - microscopy , deconvolution , light sheet fluorescence microscopy , resolution (logic) , optics , digital pathology , image resolution , materials science , sample (material) , biomedical engineering , computer science , artificial intelligence , scanning confocal electron microscopy , physics , medicine , thermodynamics
The current gold-standard histopathology for tissue analysis is destructive, time consuming, and limited to 2D slices. Light sheet microscopy has emerged as a powerful tool for 3D imaging of tissue biospecimens with its fast speed and low photo-damage, but usually with worse axial resolution and complicated configuration for sample imaging. Here, we utilized inverted selective plane illumination microscopy for easy sample mounting and imaging, and dual-view imaging and deconvolution to overcome the anisotropic resolution. We have rendered 3D images of fresh cytology cell blocks and millimeter- to centimeter-sized fixed tissue samples with high resolution in both lateral and axial directions. More accurate cellular quantification, higher image sharpness, and more image details have been achieved with the dual-view method compared with single-view imaging.