Open AccessIn vivo tomographic visualization of intracochlear vibration using a supercontinuum multifrequency-swept optical coherence microscope
Author(s) -
Samuel Choi,
Fumiaki Nin,
T. Ôta,
Kouhei Sato,
Shogo Muramatsu,
Hiroshi Hibino
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.003317
Subject(s) - supercontinuum , optical coherence tomography , optics , microscope , vibration , materials science , microscopy , coherence (philosophical gambling strategy) , amplitude , physics , acoustics , optical fiber , photonic crystal fiber , quantum mechanics
This study combined a previously developed optical system with two additional key elements: a supercontinuum light source characterized by high output power and an analytical technique that effectively extracts interference signals required for improving the detection limit of vibration amplitude. Our system visualized 3D tomographic images and nanometer scale vibrations in the cochlear sensory epithelium of a live guinea pig. The transverse- and axial-depth resolution was 3.6 and 2.7 µm, respectively. After exposure to acoustic stimuli of 21-25 kHz at a sound pressure level of 70-85 dB, spatial amplitude and phase distributions were quantified on a targeted surface, whose area was 522 × 522 μm 2 .