Open AccessQuantitative phase imaging of adherent mammalian cells: a comparative study
Author(s) -
Cédric Allier,
Lionel Hervé,
Ondřej Mandula,
Pierre Blandin,
Yves Usson,
Julien Savatier,
Serge Monneret,
Sophie Morales
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.002768
Subject(s) - digital holographic microscopy , microscopy , phase imaging , optics , optical density , lens (geology) , digital holography , live cell imaging , holography , interferometry , phase (matter) , biomedical engineering , chemistry , cell , physics , medicine , biochemistry , organic chemistry
The Quantitative phase imaging methods have several advantages when it comes to monitoring cultures of adherent mammalian cells. Because of low photo-toxicity and no need for staining, we can follow cells in a minimally invasive way over a long period of time. The ability to measure the optical path difference in a quantitative manner allows the measurement of the cell dry mass, an important metric for studying the growth kinetics of mammalian cells. Here we present and compare cell measurements obtained with three different techniques: digital holographic microscopy, lens-free microscopy and quadriwave lateral sheering interferometry. We report a linear relationship between optical volume density values measured with these different techniques and estimate the precisions of this measurement for the different individual instruments.