Open AccessLabel-free tomography of living cellular nanoarchitecture using hyperspectral self-interference microscopy
Author(s) -
Rongxin Fu,
Ya Su,
Ruliang Wang,
Lan Xue,
Kai Jiang,
Xiangyu Jin,
Han Na Yang,
Li Ma,
XueGang Luo,
Ying Lü,
Guanwen Huang
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.002757
Subject(s) - hyperspectral imaging , nanoscopic scale , microscopy , tomography , materials science , interference microscopy , interference (communication) , optics , biomedical engineering , optical microscope , optical coherence tomography , computer science , nanotechnology , artificial intelligence , medicine , telecommunications , scanning electron microscope , channel (broadcasting) , physics , composite material
Quantitative phase imaging (QPI) is the most ideal method for achieving long-term cellular tomography because it is label free and quantitative. However, for current QPI instruments, interference signals from different layers overlay with each other and impede nanoscale optical sectioning. Integrated incubators and improved configurations also require further investigation for QPI instruments. In this work, hyperspectral self-reflectance microscopy is proposed to achieve label-free tomography of living cellular nanoarchitecture. The optical description and tomography reconstruction algorithm were proposed so that the quantitative morphological structure of the entire living cell can be acquired with 89.2 nm axial resolution and 1.91 nm optical path difference sensitivity. A cell incubator was integrated to culture living cells for in situ measurement and expensive precise optical components were not needed. The proposed system can reveal native and dynamic cellular nanoscale structure, providing an alternative approach for long-term monitoring and quantitative analysis of living cells.