Open AccessIn vivo longitudinal depth-wise visualization of tumorigenesis by needle-shaped side-view confocal endomicroscopy
Author(s) -
JoongHo Ahn,
Eunji Kong,
Kibaek Choe,
Eunjoo Song,
Yoonha Hwang,
Hyun-Ji Seo,
Inwon Park,
Pilhan Kim
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.002719
Subject(s) - endomicroscopy , confocal , confocal microscopy , in vivo , pathology , preclinical imaging , carcinogenesis , biomedical engineering , microscope , materials science , biology , optics , medicine , microbiology and biotechnology , cancer , physics , genetics
In vivo , longitudinal observation of tumorigenesis in a live mouse model over an extended time period has been actively pursued to obtain a better understanding of the cellular and molecular mechanism in a highly complex tumor microenvironment. However, common intravital imaging approaches based on a conventional laser scanning confocal or a two-photon microscope have been mostly limited to the observation of superficial parts of the solid tumor tissue. In this work, we implemented a small diameter needle-shaped side-view confocal endomicroscope that can be directly inserted into a solid tumor in a minimally-invasive manner in vivo . By inserting the side-view endomicroscope into the breast tumor from the surface, we achieved in vivo depth-wise cellular-level visualization of microvasculature and fluorescently labeled tumor cells located deeply inside the tumor. In addition, we successfully performed longitudinal depth-wise visualization of a growing breast tumor over three weeks in a live mouse model, which revealed dynamic changes in microvasculature such as a decreasing amount of intratumoral blood vessels over time.