Open AccessOn-line optical clearing method for whole-brain imaging in mice
Author(s) -
Hao Wu,
Xiaoquan Yang,
Siqi Chen,
Liuyun Zhang,
Ben Long,
Chaozhen Tan,
Jing Yuan,
Hui Gong
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.002612
Subject(s) - clearing , clearance , optical imaging , optics , line (geometry) , materials science , biomedical engineering , computer science , medicine , physics , geometry , mathematics , finance , economics , urology
The combination of optical clearing with light microscopy has a number of applications in the whole-brain imaging of mice. However, the initial processing time of optical clearing is time consuming, and the protocol is complicated. We propose a novel method based on on-line optical clearing. Agarose-embedded mouse brain was immersed in the optical clearing reagent, and clearing of the brain was achieved ~100 μm beneath the sample surface. After imaging, the cleared layer was removed, thereby allowing layer-by-layer clearing and imaging. No pre-immersion was required, and we demonstrated that on-line optical clearing can reduce the whole-brain imaging time by half.