Open AccessNarrowband-autofluorescence imaging for bone analysis
Author(s) -
Laure Fauch,
Anni Palander,
Hannah Dekker,
E.A.J.M. Schulten,
Arto Koistinen,
Arja M. Kullaa,
Markku Keinänen
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.002367
Subject(s) - autofluorescence , monochrome , spectral imaging , materials science , optics , biomedical engineering , fluorescence , medicine , physics
We present a new autofluorescence-imaging method for bone analysis. This method, based on the autofluorescence of bone, provides color images in microscopic scale. The color images are created from three monochrome images acquired with optimal excitation- and emission-wavelengths combinations. The choice of these combinations were determined from the study of two-dimensional distributions of bone-features-bispectral autofluorescence in the visible- and ultraviolet-spectral range. We demonstrate that main-bone features visualized with MG-staining method can also be visualized in the autofluorescence-color image. Furthermore, the autofluorescence-color image presents features hardly distinguished in a histological-bone section.