Open AccessImaging the brain in 3D using a combination of CUBIC and immunofluorescence staining
Author(s) -
Yangyang Xu,
Peng Li,
Mengqi Wang,
Jie Zhang,
Wei Wang
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.002141
Subject(s) - staining , immunofluorescence , pathology , microscopy , high resolution , biomedical engineering , materials science , biology , medicine , antibody , immunology , remote sensing , geology
Three-dimensional (3D) high-resolution imaging can help us better understand the brain, and CUBIC is a powerful technique to render the brain transparent, thus benefiting 3D imaging. In this study, we combined CUBIC with immunofluorescence staining (IFS) and successfully acquired 3D high-resolution images of cerebral vessels, astrocytes, dopaminergic neurons and pericytes, providing us abundant information about the change in spatial frequency content for cellular scale features. Additionally, we found that a longer duration of antibody incubation produced deeper staining. Our study indicates the broad application prospects of IFS-based CUBIC for studying specific cerebral structures and diseases related to alterations of these structures.