Open AccessSurface cytometer for fluorescent detection and growth monitoring of bacteria over a large field-of-view
Author(s) -
Rafaël Sibilo,
Juan Miguel Moya Pérez,
Cédric Hurth,
Valerio Pruneri
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.002101
Subject(s) - fluorescence , detection limit , materials science , microscopy , image sensor , light emitting diode , optics , fluorescence microscope , diode , optoelectronics , semiconductor , nanotechnology , chemistry , physics , chromatography
Monitoring the early onset of bacterial film formation is critical in many clinical, environmental, and food quality control applications. We built a small inexpensive optical surface cytometer, in contrast with bulk spectroscopic methods, around a light-emitting diode (LED) and a complementary metal-oxide-semiconductor (CMOS) image sensor. It is designed to offer a large field-of-view of 200 mm 2 and a large depth-of-field of 2-3 mm to overcome the limitations of routine methods like spectrophotometry and fluorescence microscopy. It provides a direct measurement without the need for complex image post-processing with a limit-of-detection around 10 4 cells/mm 2 , which is competitive with other similar yet more complex devices already available.