Open AccessMulti-wavelength quantitative polarization and phase microscope
Author(s) -
Xiaobo Tian,
Xingzhou Tu,
Kimiko Della Croce,
Guang Yao,
Haijiang Cai,
Neal Brock,
Stanley Pau,
Rongguang Liang
Publication year - 2019
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.10.001638
Subject(s) - microscope , optics , polarization (electrochemistry) , birefringence , microscopy , materials science , polarized light microscopy , optical microscope , wavelength , polarization microscopy , scanning electron microscope , physics , chemistry
We introduce a snapshot multi-wavelength quantitative polarization and phase microscope (MQPPM) for measuring spectral dependent quantitative polarization and phase information. The system uniquely integrates a polarized light microscope and a snap-shot quantitative phase microscope in a single system, utilizing a novel full-Stokes camera operating in the red, green, and blue (RGB) spectrum. The linear retardance and fast axis orientation of a birefringent sample can be measured simultaneously in the visible spectra. Both theoretical analysis and experiments have been performed to demonstrate the capability of the proposed microscope. Data from liquid crystal and different biological samples are presented. We believe that MQPPM will be a useful tool in measuring quantitative polarization and phase information of live cells.