Optical properties of adult Drosophila brains in one-, two-, and three-photon microscopy | Zendy

Kuo-Jen Hsu | Zendy; Yen-Yin Lin | Zendy; AnnShyn Chiang | Zendy; ShiWei Chu | Zendy

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Optical properties of adult Drosophila brains in one-, two-, and three-photon microscopy

Author(s) -

Kuo-Jen Hsu,

Yen-Yin Lin,

AnnShyn Chiang,

ShiWei Chu

Publication year - 2019

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.10.001627

Subject(s) - optics , scattering , two photon excitation microscopy , light scattering , wavelength , connectome , distortion (music) , photon , excitation , microscopy , physics , materials science , optoelectronics , biology , neuroscience , fluorescence , amplifier , cmos , quantum mechanics , functional connectivity

Drosophila is widely used in connectome studies due to its small brain size, sophisticated genetic tools, and the most complete single-neuron-based anatomical brain map. Surprisingly, even the brain thickness is only 200-μm, common Ti:sapphire-based two-photon excitation cannot penetrate, possibly due to light aberration/scattering of trachea. Here we quantitatively characterized scattering and light distortion of trachea-filled tissues, and found that trachea-induced light distortion dominates at long wavelength by comparing one-photon (488-nm), two-photon (920-nm), and three-photon (1300-nm) excitations. Whole- Drosophila -brain imaging is achieved by reducing tracheal light aberration/scattering via brain-degassing or long-wavelength excitation at 1300-nm. Our work paves the way toward constructing whole-brain connectome in a living Drosophila .

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