Open AccessImaging effectiveness calculator for non-design microscope samples
Author(s) -
Stephen M. Anthony,
Philip R. Miller,
Jerilyn A. Timlin,
Ronen Polsky
Publication year - 2019
Publication title -
applied optics
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 197
eISSN - 2155-3165
pISSN - 1559-128X
DOI - 10.1364/ao.58.006027
Subject(s) - strehl ratio , microfabrication , optics , microscope , materials science , microfluidics , image quality , refractive index , signal to noise ratio (imaging) , fabrication , microscopy , computer science , nanotechnology , optoelectronics , adaptive optics , physics , artificial intelligence , medicine , alternative medicine , pathology , image (mathematics)
When attempting to integrate single-molecule fluorescence microscopy with microfabricated devices such as microfluidic channels, fabrication constraints may prevent using traditional coverslips. Instead, the fabricated devices may require imaging through material with a different thickness or index of refraction. Altering either can easily reduce the quality of the image formation (measured by the Strehl ratio) by a factor of 2 or more, reducing the signal-to-noise ratio accordingly. In such cases, successful detection of single-molecule fluorescence may prove difficult or impossible. Here we provide software to calculate the effect of non-design materials upon the Strehl ratio or ensquared energy and explore the impact of common materials used in microfabrication.