Open AccessVisualizing bleb mass dynamics in single cells using quantitative phase microscopy
Author(s) -
Zachary A. Steelman,
Anna Sedelnikova,
Zachary Coker,
Allen S. Kiester,
Gary D. Noojin,
Bennett L. Ibey,
Joel N. Bixler
Publication year - 2021
Publication title -
applied optics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 197
eISSN - 2155-3165
pISSN - 1559-128X
DOI - 10.1364/ao.426147
Subject(s) - optics , materials science , microscopy , bleb (medicine) , phase imaging , live cell imaging , optical path length , biophysics , chemistry , physics , cell , medicine , intraocular pressure , biology , biochemistry , trabeculectomy , ophthalmology
Understanding biological responses to directed energy (DE) is critical to ensure the safety of personnel within the Department of Defense. At the Air Force Research Laboratory, we have developed or adapted advanced optical imaging systems that quantify biophysical responses to DE. One notable cellular response to DE exposure is the formation of blebs, or semi-spherical protrusions of the plasma membrane in living cells. In this work, we demonstrate the capacity of quantitative phase imaging (QPI) to both visualize and quantify the formation of membrane blebs following DE exposure. QPI is an interferometric imaging tool that uses optical path length as a label-free contrast mechanism and is sensitive to the non-aqueous mass density, or dry mass, of living cells. Blebs from both CHO-K1 and U937 cells were generated after exposure to a series of 600 ns, 21.2 kV/cm electric pulses. These blebs were visualized in real time, and their dry mass relative to the rest of the cell body was quantified as a function of time. It is our hope that this system will lead to an improved understanding of both DE-induced and apoptotic blebbing.