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Reduction of Osteoclasts in a Critical Embryonic Period Is Essential for Inhibition of Mouse Tooth Eruption
Author(s) -
Yoshino Miya,
Yamazaki Hidetoshi,
Yoshida Hisahiro,
Niida Shumpei,
Nishikawa ShinIchi,
Ryoke Kazuo,
Kunisada Takahiro,
Hayashi ShinIchi
Publication year - 2003
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1359/jbmr.2003.18.1.108
Subject(s) - tooth eruption , osteoclast , osteopetrosis , endocrinology , dental alveolus , bone resorption , macrophage colony stimulating factor , medicine , bone remodeling , resorption , tartrate resistant acid phosphatase , antibody , immunology , biology , receptor , macrophage , dentistry , molar , biochemistry , in vitro
Alveolar bone resorption by osteoclasts is essential for tooth eruption. Osteoclast‐deficient Csfm op homozygous ( op/op ) mice, which lack functional macrophage colony‐stimulating factor (M‐CSF), suffer from osteopetrosis and completely lack tooth eruption. Although osteoclasts appear, and osteopetrosis is cured with age in op/op mice, tooth eruption is never seen. This fact suggests that there is a critical period when osteoclasts are required for tooth eruption. In this study, to detect the critical period, we administered an antagonistic antibody directed against c‐Fms, a receptor for M‐CSF, to inbred C57BL/6 mice for various periods. Administration of this antibody decreased tartrate‐resistant acid phosphatase‐positive (TRAP) osteoclasts, and incisor eruption was completely inhibited by continual administration of this antibody from embryonic day 15.5 (E15.5) until postnatal day 12.5 (D12.5). A 1‐day delay of this administration abolished the inhibition of incisor eruption. The number of TRAP‐positive osteoclasts was significantly reduced between E16.5 and E18.5 in the mice treated with antibody from E15.5 compared with those treated from E16.5. These results indicate that this period, during which the number of osteoclasts decreases significantly, is critical for inhibiting incisor eruption in C57BL/6 mice.