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Three‐Dimensional‐Line Skeleton Graph Analysis of High‐Resolution Magnetic Resonance Images: A Validation Study From 34‐μm‐Resolution Microcomputed Tomography
Author(s) -
Pothuaud Laurent,
Laib Andres,
Levitz Pierre,
Benhamou Claude L.,
Majumdar Sharmila
Publication year - 2002
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1359/jbmr.2002.17.10.1883
Subject(s) - skeleton (computer programming) , tomography , magnetic resonance imaging , osteoporosis , smoothing , trabecular bone , high resolution , materials science , biomedical engineering , anatomy , computer science , computer vision , radiology , medicine , pathology , geology , remote sensing
The resolution achievable in vivo by magnetic resonance imaging (MRI) techniques is not sufficient to depict precisely individual trabeculae and, thus, does not permit the quantification of the “true” trabecular bone morphology and topology. Nevertheless, the characterization of the “apparent” trabecular bone network derived from high‐resolution MR images (MRIs) and their potential to provide information in addition to bone mineral density (BMD) alone has been established in studies of osteoporosis. The aim of this work was to show the ability of the three‐dimensional‐line skeleton graph analysis (3D‐LSGA) to characterize high‐resolution MRIs of trabecular bone structure. Fifteen trabecular bone samples of the distal radius were imaged using the high‐resolution MRI (156 × 156 × 300 μm 3 ) and microcomputed tomography (μCT; 34 × 34 × 34 μm 3 ). After thresholding, the 3D skeleton graph of each binary image was obtained. To remove the assimilated‐noise branches of the skeleton graph and smooth this skeleton graph before it was analyzed, we defined a smoothing length criterion ( l c ), such that all “termini” branches having a length lower than l c were removed. Local topological and morphological LSGA measurements were performed from MRIs and μCT images of the same samples. The correlations between these two sets of measurements were dependent on the smoothing criterion l c , reaching R 2 = 0.85 for topological measurements and R 2 = 0.57–0.64 for morphological measurements. 3D‐LSGA technique could be applied to in vivo high‐resolution MRIs of trabecular bone structure, giving an indirect characterization of the microtrabecular bone network.