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Study of the Maturation of the Organic (Type I Collagen) and Mineral (Nonstoichiometric Apatite) Constituents of a Calcified Tissue (Dentin) as a Function of Location: A Fourier Transform Infrared Microspectroscopic Investigation
Author(s) -
Magne David,
Weiss Pierre,
Bouler JeanMichel,
Laboux Olivier,
Daculsi Guy
Publication year - 2001
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1359/jbmr.2001.16.4.750
Subject(s) - mineralized tissues , mineralization (soil science) , apatite , dentin , enamel paint , chemistry , biomineralization , mineral , fourier transform infrared spectroscopy , infrared spectroscopy , biophysics , mineralogy , materials science , chemical engineering , organic chemistry , composite material , biology , nitrogen , engineering
Fourier transform infrared microspectroscopy (FTIRM) was used to investigate the organic and mineral phases of a calcified tissue (dentin) as a function of its location from predentin toward enamel. Thin dentin slices (decalcified or not) were fixed in formaldehyde and embedded in glycolmethylmethacrylate (GMA). Fixation did not denature collagen, and GMA did not interact with organic or mineral constituents of dentin. The ν 1 ν 3 PO 4 domain was studied in particular in order to estimate mineral maturity and amide I, II, A, and B to obtain data on protein conformation. The results showed that dentin apatite became increasingly mature (stoichiometric) from the mineralization front toward the enamel, especially through loss of HPO 4 2− groups and vacancies. Moreover, collagen fibrils became less and less hydrated, suggesting that intrafibrillar mineralization partially dehydrated the collagen. Combined study of the organic and mineral fractions of calcified tissues may help clarify their relationships in physiological and pathological tissues.