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Effect of Prostaglandin and Bisphosphonate on Cancellous Bone Volume and Structure in the Ovariectomized Rat Studied by Quantitative Three‐Dimensional Nuclear Magnetic Resonance Microscopy
Author(s) -
Takahashi Masaya,
Wehrli Felix W.,
Wehrli Suzanne L.,
Hwang Scott N.,
Lundy Mark W.,
Hartke Jim,
Borah Babul
Publication year - 1999
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1359/jbmr.1999.14.5.680
Subject(s) - ovariectomized rat , cancellous bone , bisphosphonate , chemistry , etidronic acid , nuclear medicine , osteoporosis , medicine , endocrinology , anatomy , estrogen
The purpose of this work was to evaluate the potential of nuclear magnetic resonance microscopy (NMRM) in conjunction with a processing technique to monitor the effect of preventive agents in an ovariectomized (OVX) rat. Twenty‐five female Sprague‐Dawley rats were OVX at 6 months of age (except for the intact control group), allowed to lose bone for 60 days, and then treated for 60 days. During treatment, animals were administered vehicle, prostaglandin E 2 (PGE 2 ; 6 mg/kg), or alendronate (3 μg/kg) subcutaneously once a day. Subsequently, tibiae were harvested and the marrow removed. NMRM was carried out at 9.4 T, with the specimens immersed in 1.2 mM diethylenetriaminepentaacetic acid‐gadolinium salt (Gd‐DTPA) aqueous solution. A three‐dimensional (3D) partial flip‐angle pulse sequence was used, providing a 128 3 array of (46 μm) 3 isotropic voxels. Fifty of the 128 axial images in the 3D data set comprising ∼2.4 mm volume distal to the growth plate were processed from each specimen using a probability‐based method for determining bone volume fraction (BVF), tubularity, contiguity, as well as the mean trabecular plate thickness and separation. PGE 2 and alendronate altered BVF consistently at all tibial regions. The effect of alendronate was to keep BVF about midway between intact and OVX, whereas PGE 2 returned BVF to intact levels. The other parameters showed similar responses to treatment. The strongest discriminator was trabecular BVF, which could obviously differentiate the groups. The study establishes NMRM as a nondestructive histomorphometric method for the quantitative evaluation of drug response in a rat ovariectomy model.