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The Chemokine Interleukin‐8 Regulates Parathyroid Secretion
Author(s) -
Angeletti Ruth Hogue,
D'Amico Thomas,
Ashok Sheela,
Russell John
Publication year - 1998
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1359/jbmr.1998.13.8.1232
Subject(s) - parathyroid hormone , chemokine , parathyroid hormone receptor , microbiology and biotechnology , receptor , medicine , endocrinology , biology , messenger rna , expression cloning , parathyroid chief cell , complementary dna , chemistry , gene , calcium , hormone receptor , biochemistry , cancer , breast cancer
Interleukin‐8 (IL‐8) is a chemokine important in inflammatory processes. Homology cloning experiments performed using bovine parathyroid cDNA and degenerate primers encoding transmembrane regions III and VI of peptide and protein hormone G‐protein coupled receptors identified a set of known receptors not previously identified in the parathyroid. Among these was the IL‐8 type B receptor. Incubation of freshly isolated bovine parathyroid cells with recombinant IL‐8 for 6–48 h produced an increase in the levels of mRNA for parathyroid hormone (PTH). The levels of PTH secreted in response to nanomolar amounts of IL‐8 were also elevated in cells incubated for 1 h with IL‐8. Differential display analysis of mRNA from parathyroid cells, incubated in the presence and absence of IL‐8, permitted the identification of cDNA clones for RNA species whose expression was either elevated or suppressed. These experiments suggest that IL‐8 and inflammatory events play a role in bone homeostasis through actions on the parathyroid gland.