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A New Method to Isolate Large Numbers of Rabbit Osteoclasts and Osteoclast‐like Cells: Application to the Characterization of Serum Response Element Binding Proteins During Osteoclast Differentiation
Author(s) -
David JeanPierre,
Neff Lynn,
Chen Yan,
Rincon Mercedes,
Horne William C.,
Baron Roland
Publication year - 1998
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1359/jbmr.1998.13.11.1730
Subject(s) - osteoclast , cellular differentiation , serum response factor , biology , microbiology and biotechnology , in vitro , function (biology) , transcription factor , biochemistry , gene
We have developed a new method that allows the purification of large numbers of both authentic osteoclasts (OCs) and in vitro differentiated osteoclast‐like cells (OCLs) from rabbits. We characterized the OCLs in terms of the expression of different phenotypic markers of OC differentiation and their ability to resorb bone. The method provides a system for performing biochemical and molecular studies of OC differentiation and function in a single species. We used this system to characterize the effect of 1,25‐dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) on the expression of proteins that bind to the serum response element (SRE) of the c‐ fos promoter. We found that OCLs and OCs displayed similar SRE‐binding activities, including the serum response factor (SRF). This pattern is established in a time‐dependent and cell‐specific manner in response to long‐term treatment of rabbit bone marrow by 1,25(OH) 2 D 3 . Thus, 1,25(OH) 2 D 3 can modulate SRF and/or SRF‐related protein. This finding may contribute to understanding the role of c‐Fos in the regulation of OC differentiation.