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Identifying a molecular phenotype for bone marrow stromal cells with in vivo bone‐forming capacity
Author(s) -
Larsen Kenneth H,
Frederiksen Casper M,
Burns Jorge S,
Abdallah Basem M,
Kassem Moustapha
Publication year - 2010
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1359/jbmr.091018
Subject(s) - bone sialoprotein , bone marrow , osteopontin , stromal cell , in vivo , osteocalcin , runx2 , pathology , biology , microbiology and biotechnology , chemistry , osteoblast , alkaline phosphatase , cancer research , immunology , in vitro , medicine , biochemistry , genetics , enzyme
The ability of bone marrow stromal cells (BMSCs) to differentiate into osteoblasts is being exploited in cell‐based therapy for repair of bone defects. However, the phenotype of ex vivo cultured BMSCs predicting their bone‐forming capacity is not known. Thus we employed DNA microarrays comparing two human bone marrow stromal cell (hBMSC) populations: One is capable of in vivo heterotopic bone formation (hBMSC‐TERT +Bone ), and the other is not (hBMSC‐TERT –Bone ). Compared with hBMSC‐TERT – Bone , the hBMSC‐TERT +Bone cells had an increased overrepresentation of extracellular matrix genes (17% versus 5%) and a larger percentage of genes with predicted SP3 transcription factor–binding sites in their promoter region (21% versus 8%). On the other hand, hBMSC‐TERT – Bone cells expressed a larger number of immune‐response‐related genes (26% versus 8%). In order to test for the predictive value of these markers, we studied the correlation between their expression levels in six different hBMSC‐derived clones and the ability to form bone in vivo. We found a significant correlation for decorin, lysyl oxidase‐like 4, natriuretic peptide receptor C, and tetranectin. No significant positive correlation was found for canonical osteoblastic markers Runx2, alkaline phosphatase, collagen type I, osteopontin, and bone sialoprotein. Prospective isolation of four additional hBMSC clones based on their expression levels of the molecular markers correlated with their in vivo bone‐formation ability. In conclusion, our data suggest an in vitro molecular signature predictive for hBMSCs' in vivo bone‐formation ability. Identifying more of these predictive markers would be very useful in the quality control of osteoblastic cells before use in therapy. © 2010 American Society for Bone and Mineral Research