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A Novel Mutation (K378X) in the Sequestosome 1 Gene Associated With Increased NF‐κB Signaling and Paget's Disease of Bone With a Severe Phenotype
Author(s) -
Rea Sarah L,
Walsh John P,
Ward Lynley,
Yip Kirk,
Ward Bryan K,
Kent G Neil,
Steer James H,
Xu Jiake,
Ratajczak Thomas
Publication year - 2006
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1359/jbmr.060405
Subject(s) - sequestosome 1 , osteoclast , paget's disease of bone , mutation , bone resorption , transfection , mutant , biology , luciferase , wild type , cancer research , microbiology and biotechnology , genetics , cell culture , gene , medicine , autophagy , in vitro , disease , apoptosis
Abstract Sequestosome 1 /p62 (p62) mutations are associated with PDB; however, there are limited data regarding functional consequences. We report a novel mutation in exon 7 (K378X) in a patient with polyostotic Paget's disease of bone. p62 mutants increased NF‐κB activation and significantly potentiated osteoclast formation and bone resorption in human primary cell cultures. Introduction: Sequestosome 1 /p62 (p62) mutations are associated with Paget's disease of bone (PDB); however, there are limited data regarding functional consequences. One report has linked the common P392L mutation in the p62 ubiquitin binding associated (UBA) domain with increases in NF‐κB activity, a transcription factor essential for osteoclastogenesis. To further clarify the functional impact of p62 mutations associated with PDB, we assessed the effect of p62 mutation (a novel mutation: K378X, and previously reported mutations: P392L and E396X) on RANK‐induced NF‐κB activation and compared this with the effect of wildtype p62. In addition, we studied the effect of p62 mutation on osteoclast formation and bone resorption. Materials and Methods: We performed co‐transfection experiments with expression plasmids for p62 (wildtype or mutated) and RANK and an NF‐κB luciferase reporter gene. Luciferase activities were recorded after addition of luciferin to cellular lysates. RAW 264.7 cells stably expressing enhanced green fluorescent protein (EGFP)‐tagged p62 (wildtype, K378X, or P392L) or EGFP alone were assessed for changes in cell proliferation. Additionally, these cells were stimulated with RANKL to produce osteoclast‐like cells (OLCs). Primary human monocytes collected from the K378X‐affected patient and a control subject were stimulated to form OLCs and bone resorption data were obtained. Results: The novel mutation introduces a premature stop codon in place of Lys‐378 and thereby eliminates the entire p62 UBA domain; this and two additional natural mutations (P392L, E396X) increased NF‐κB activation compared with wildtype p62. Wildtype p62 consistently inhibited NF‐κB activation compared with empty vector. UBA mutations (K378X and P392L) significantly increased the number of OLCs formed in response to RANKL and also the number of nuclei of the OLCs. K378X‐affected human monocytes formed more OLCs with more nuclei and increased bone resorption compared with control monocytes. Conclusions: Our data show that mutation of the p62 UBA domain results in increased activation of NF‐κB and osteoclast formation and function compared with wildtype p62. These results may partially explain the mechanism by which p62 mutation contributes to the pathogenesis of PDB.