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Inhibition of RANKL‐Induced Osteoclastogenesis by (−)‐DHMEQ, a Novel NF‐κB Inhibitor, Through Downregulation of NFATc1
Author(s) -
Takatsuna Hiroshi,
Asagiri Masataka,
Kubota Takeshi,
Oka Kotaro,
Osada Toshihiro,
Sugiyama Chie,
Saito Hiroaki,
Aoki Kazuhiro,
Ohya Keiichi,
Takayanagi Hiroshi,
Umezawa Kazuo
Publication year - 2005
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1359/jbmr.041213
Subject(s) - rankl , downregulation and upregulation , osteoclast , multinucleate , cancer research , chemistry , nf κb , microbiology and biotechnology , transcription factor , osteolysis , signal transduction , biology , receptor , activator (genetics) , medicine , biochemistry , gene , surgery
Abstract (−)‐DHMEQ, a newly designed NF‐κB inhibitor, inhibited RANKL‐induced osteoclast differentiation in mouse BMMs through downregulation of the induction of NFATc1, an essential transcription factor of osteoclastogenesis. Introduction: Bone destruction is often observed in advanced case of rheumatoid arthritis and neoplastic diseases, including multiple myeloma. Effective and nontoxic chemotherapeutic agents are expected for the suppression of these bone destructions. RANKL induces activation of NF‐κB and osteoclastogenesis in bone marrow‐derived monocyte/macrophage precursor cells (BMMs). Targeted disruption or pharmacological suppression of NF‐κB result in impaired osteoclastogenesis, but how NF‐κB is involved in the regulation of osteoclastogenesis is not known. Materials and Methods: The effect of (−)‐dehydroxymethylepoxyquinomicin [(−)‐DHMEQ] on osteoclast differentiation was studied using a culture system of mouse BMMs stimulated with RANKL and macrophage colony‐stimulating factor. The mechanism of the inhibition was studied by biochemical analysis such as immunoblotting and retroviral transfer experiments. Results: (−)‐DHMEQ strongly inhibited RANKL‐induced NF‐κB activation in BMMs and inhibited RANKL‐induced formation of TRACP + multinucleated cells. Interestingly, (−)‐DHMEQ specifically inhibited the RANKL‐induced expression of NFATc1 but not the expressions of TRAF6 or c‐ fos . Inhibition of osteoclast differentiation by (−)‐DHMEQ was rescued by overexpression of NFATc1, suggesting that the inhibition is not caused by a toxic effect. Moreover, pit formation assays showed that (−)‐DHMEQ also inhibited the bone‐resorbing activity of mature osteoclasts. Conclusion: The inhibition of NF‐κB suppresses osteoclastogenesis by downregulation of NFATc1, suggesting that NFATc1 expression is regulated by NF‐κB in RANKL‐induced osteoclastogenesis. Our results also indicate the possibility of (−)‐DHMEQ becoming a new therapeutic strategy against bone erosion.