Open AccessProduction and Characterization of Crude Protease from RS1 Isolate from silage of Floating Bladderwort (Utricularia gibba)
Author(s) -
Ace Baehaki,
Arif Hidayat,
Nuni Gofar,
Rodiaopianti
Publication year - 2020
Publication title -
mediterranean journal of chemistry
Language(s) - English
Resource type - Journals
ISSN - 2028-3997
DOI - 10.13171/mjc02003251256ab
Subject(s) - protease , chemistry , silage , molecular mass , swamp , chromatography , enzyme , biochemistry , food science , biology , ecology
The purpose of this research was to produce and characterizing crude protease from RS1 isolate of swamp plant silage. The optimum production time of RS1 isolate was 40 h. The optimum pH and temperature of protease from RS1 isolate were 10 and 45℃, respectively. Ion Mg3+ increased RS1 protease whereas ion of Na+, K+, Fe2+, and Zn2+ inhibited protease from RS1 isolate. Study on the effect of metals ion indicated that protease from RS1 isolate was metaloenzyme. Based analysis on SDS-PAGE, the molecular weight of RS1 protease had 12 bands with molecular weights ranging from 34.75 kDa to 263.53 kDa.