Open AccessPotensi limbah kulit buah Nyirih Xylocarpus granatum sebagai inhibitor tirosinase
Author(s) -
Mohamad Gazali,
Neviaty P. Zamani,
Irmanida Batubara
Publication year - 2014
Publication title -
depik
Language(s) - English
Resource type - Journals
eISSN - 2502-6194
pISSN - 2089-7790
DOI - 10.13170/depik.3.3.2153
Subject(s) - chemistry , chloroform , tyrosinase , maceration (sewage) , hexane , ethyl acetate , methanol , dichloromethane , chromatography , ic50 , food science , traditional medicine , enzyme , organic chemistry , biochemistry , solvent , in vitro , materials science , composite material , medicine
The aim of the present study was to analyse the peel waste of Xylocarpus granatum fruits as potential source of tyrosinase inhibitors. Dried peel samples were ground to yield a powder (simplicia). Subsequently, they were extracted with organic solvents of distinct polarity levels, namely n-hexane (non-polar), chloroform (semi-polar) and methanol (polar) by use of the single-maceration method. Inhibitory effects on tyrosinase activity (monophenolase) and DOPA auto-oxidation (diphenolase) were determined in bioassays. Assays with the methanol extract revealed IC50 values of 784.87 μg mL-1 (monophenolase) and of 1176.66 μg mL-1 (diphenolase), respectively. In contrast, n-hexane and chloroform extracts showed no activity. These results indicate that the methanolic fruit peel extract contained tyrosinase-inhibiting compounds, such as flavonoids, tannins and saponins, whereas the n-hexane and chloroform extracts yielded alkaloids, steroids and triterpenoids without tyrosinase-inhibiting activity. The phenolic compounds had a strong effect on the tyrosinase enzymes, inhibiting monophenolases by 97% and diphenolases by 96%, with a positive correlation between the total phenolic content and the inhibition rate in both activities.