Open AccessConservação in vitro de Erythrina velutina a partir de embriões zigóticos
Author(s) -
Priscila Tavares Fonseca,
Cristina Ferreira Nepomuceno,
Tecla dos Santos Silva,
José Raniere Ferreira de Santana
Publication year - 2015
Publication title -
sitientibus. série ciências biológicas
Language(s) - English
Resource type - Journals
eISSN - 2238-4103
pISSN - 1519-6097
DOI - 10.13102/scb737
Subject(s) - biology , shoot , explant culture , germination , botany , inoculation , callus , embryo , horticulture , in vitro , biochemistry , microbiology and biotechnology
Erythrina velutina is native from the Caatinga domain, which has been heavily affected by the exploitation of natural resources. Thus, the aim of this study was to evaluate the induction of buds from nodal segments of in vitro germinated plants, aiming at the conservation of E. velutina. In the experiment I, intact seeds and zygotic embryos were inoculated in WPM culture and the number of buds, leaves and roots, germination percentage, shoot length and percentage of normal plants were analyzed. In the experiment II, the nodal segments of plants from germination of zygotic embryos in vitro were inoculated in WPM culture supplemented with BAP and CIN [0.0, 10.0, 20.0, 30.0 and 40.0 µM] and percentage of responsive explants to form buds, number of shoots and leaves, shoot length and the percentage of callus formation were analyzed. The cultivation of zygotic embryos provided more vigorous and uniform plants, with more gems than those obtained from the germination of intact seeds. The concentration of 20 µM of BAP is indicated for the regeneration of plants from zygotic embryos of E. velutina; with this concentration, an average of 2.78 shoots/explant was obtained.