Open AccessMultiplicação in vitro de Caesalpinia pyramidalis (Leguminosae)
Author(s) -
Tecla dos Santos Silva,
Cristina Ferreira Nepomuceno,
Bárbara Paula dos Santos Borges,
Bruno Freitas Matos Alvim,
José Raniere Ferreira de Santana
Publication year - 2013
Publication title -
sitientibus. série ciências biológicas
Language(s) - English
Resource type - Journals
eISSN - 2238-4103
pISSN - 1519-6097
DOI - 10.13102/scb320
Subject(s) - shoot , inoculation , caesalpinia , agar , nodal , botany , biology , fodder , murashige and skoog medium , horticulture , in vitro , tissue culture , biochemistry , genetics , anatomy , bacteria
Caesalpinia pyramidalis is a species endemic to the Caatinga and known popularly as catingueira, which is widely used by local people, mainly for its timber and medicinal and fodder properties. This study investigated the effects of different types and concentrations of plant growth regulators on the in vitro multiplication of C. pyramidalis. In the first experiment, nodal segments were inoculated in media containing different combinations (0.0–8.0 µM) of BAP and NAA. In the second experiment, nodal segments wereinoculated in media containing different types (KIN, BAP and TDZ) and concentrations (0.0–16μM) of cytokinins. We used a WPM medium supplemented with 87.64 mM sucrose and solidified with 7.0 g L-1 agar. After 45 days, the highest number of shoots, leaf number, shoot length and dry mass of shoots were obtained when nodal segments were inoculated into a culture medium without plant growth regulators.