Open AccessPlant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americana bulb extract and its characterization
Author(s) -
Julinda Romauli Manullang,
Rudy Agung Nugroho,
Miftakhur Rohmah,
Rudianto Rudianto,
AMANDA QORYSUCHI
Publication year - 2021
Publication title -
nusantara bioscience
Language(s) - English
Resource type - Journals
eISSN - 2087-3956
pISSN - 2087-3948
DOI - 10.13057/nusbiosci/n130216
Subject(s) - fourier transform infrared spectroscopy , silver nanoparticle , bulb , nuclear chemistry , chemistry , scanning electron microscope , transmission electron microscopy , nanoparticle , analytical chemistry (journal) , amorphous solid , materials science , nanotechnology , botany , chemical engineering , crystallography , organic chemistry , engineering , composite material , biology
. Manullang JR, Nugroho RA, Rohmah M, Rudianto, Qorysuchi A. 2021. Plant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americana bulb extract and its characterization. Nusantara Bioscience 13: 247-254. The plant-based biosynthesis of nanoparticles has gained increasing momentum due to being lower in cost and eco-friendly. This study aimed to biosynthesize nanoparticles from the bulb ethanolic extract of Eleutherine americana (Aubl.) Merr. ex K.Heyne (Ea-AgNPs), then characterize Ea-AgNPs and determine their phytochemical content and antioxidant capacity. The Ea-AgNPs were synthesized using ethanolic extract of E. americana bulb along with various concentrations of AgNO3 (0.5-4 mM). The Ea-AgNPs were then characterized using UV-VIS spectroscopic, Scanning Electron Microscopy/ Energy Dispersive X-ray (SEM/EDX), Transmission Electron Microscopy (TEM), X-ray Powder Diffractometry (XRD), and Fourier-Transform Infrared Spectroscopy (FTIR) techniques. The results indicated that E. americana can be used to reduce AgNO3 to synthesize Ea-AgNPs, indicated by color change, and had optimum UV/VIS spectra at 400 nm. The FTIR analysis found that Ea-AgNPs showed peaks at 2919, 2850, 1586, and 1031 cm1, containing several important biocompounds. Additionally, the XRD results found an amorphous Ea-AgNP peak with maximum intensity and proportion of silver occurring at 24 Theta. The particle size distribution curve of Ea-AgNPs showed a size of 105 nm. Furthermore, SEM/EDX analysis revealed an optical absorption characteristic peak at 3 keV. The EDX examination revealed three signals: a strong signal from the C atom (70.99 %), an O atom (28.95 %), and Ag atom (0.06 %). The TEM imaging also showed the characteristics of Ea-AgNPs. Some phytochemicals such as flavonoids, tannins, alkaloids, and saponins were found in Ea-AgNPs, with IC50 values of 45.30 ppm.