Goramy spermatozoa quality after sub-zero freezing: The role of coconut water as the cryoprotectant

Abinawanto, Putri PE. 2017. Goramy spermatozoa quality after sub-zero freezing: The role of coconut water as thecryoprotectant. Cell Biol Dev 1: 1-5. The coconut water effect combined with 5% of glycerol for preserving goramy spermatozoa at -34°C for 48 hours has been studied. The objective of study is to find the best combination among 0%, 21%, 23%, 25% 27%, and 29%,respectively, of coconut water combined with 5% of glycerol for maintaining the good spermatozoa motility and viability, andminimizing spermatozoa abnormality. One part of semen/sperm were mixed with three parts of solvent (5% of glycerol + fish ringer +coconut water), and were equilibrated at 4 °C for 45 min. The diluted sperm were then freezed at -34 °C for 48 h. Cryopreserved spermswere thawed at 30 °C for 3-5 min. Spermatozoa quality were evaluated before and after sub-zero freezing. Based on Kruskal-Wallis test,spermatozoa motility and viability were higher than control (P 0.05). Twenty five percent of coconut water combined with 5% of glycerol were the best combinationfor preserving spermatozoa motility (80.36±1.54)% and spermatozoa viability (82±1.86)%, and also minimized spermatozoaabnormality (10±1.03)%.