Open AccessShort Communication: Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples
Author(s) -
Alfi Sophian,
Ratna Purwaningsih,
Muindar Muindar,
Eka Putri Juniarti Igirisa,
Muhammad Luthfi Amirullah
Publication year - 2021
Publication title -
asian journal of tropical biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2775-4715
pISSN - 2775-300X
DOI - 10.13057/biotek/c180103
Subject(s) - absorbance , extraction (chemistry) , dna , dna extraction , yield (engineering) , chromatography , gene , biology , microbiology and biotechnology , analytical chemistry (journal) , chemistry , genetics , materials science , polymerase chain reaction , metallurgy
. Sophian A, Purwaningsih R, Muindar, Igirisa EPJ, Amirullah ML. 2021. Short Communication: Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples. Asian J Trop Biotechnol 18: 28-31. The purpose of this study was to optimize the Intron patho gene-spin DNA/RNA extraction kit on crab processed food products so that it can be used for testing samples other than viral and bacterial samples. The method used in purity and concentration analysis to analyze total DNA (yield) is the absorbance method using a nanophotometer and the method used to isolate the sample DNA is the centrifuge Column or spin column method. The research data showed that the extracted sample concentration was in the range of 15.15-15.75, with an average of 15.46. while the purity values measured at the A260/A280 wavelengths were obtained with a purity range between 2,124-2,346. The total DNA analysis (yield) results obtained an average of 755.04 with the lowest yield value of 575.50 and the highest yield value of 787.50. As a suggestion for further research, it is hoped that modification can be made to perfect the isolated DNA so that it has better purity and concentration values.