Open AccessThe influence of aqueous extract of roselle (Hibiscus sabdariffa) calyx on blood cholesterol level and body weight in rats
Author(s) -
Meisa Marsalina,
Samigun Samigun,
Endang Sri Hardjanti
Publication year - 2011
Publication title -
biofarmasi
Language(s) - English
Resource type - Journals
ISSN - 1693-2242
DOI - 10.13057/biofar/f090203
Subject(s) - hibiscus sabdariffa , calyx , body weight , aqueous extract , cholesterol , chemistry , analysis of variance , zoology , in vivo , triglyceride , traditional medicine , biology , medicine , food science , biochemistry , botany , endocrinology , microbiology and biotechnology
Marsalina M, Samigun, Hardjanti ES. 2011. The influence of aqueous extract of roselle (Hibiscus sabdariffa) calyx on blood cholesterol level and body weight in rats. Biofarmasi 9: 43-49. This study aimed to investigate the influence of aqueous extract of roselle calyx on blood cholesterol level and body weight in rats. This experimental research was arranged as a pre and post-test controlled group design. Thirty Sprague-Dawley rats, with 3 months of age and 250 g in average weight, were used as an animal model. Those rats were grouped equally into five groups, i.e. negative control (aquadest), positive control (0.26 mg lovastatin/200 g body weight of rats/2 mL), the first dose of aqueous extract of roselle calyx (65 mg/200 g/2 mL), the second dose of aqueous extract of roselle calyx (130 mg/200 g/2 mL), and the third dose of aqueous extract of roselle calyx (195 mg/200 g/2 mL). The measurement of rat blood cholesterol level was conducted before and after treatment, while the rat body weight was measured once a week. The result of this study was analyzed by using one-way ANOVA statistical test. The one-way ANOVA statistical test showed no significant difference on reducing blood cholesterol level with p=0.327 (p>0.05) and body weight in rat with p=0.154, p=0.214, and p=0.938 (p>0.05). This study concluded that the aqueous extract of roselle calyx has no influence on lowering blood cholesterol level and body weight in rats.